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DNA Copy Number Calculator

Chemistry & Biology

Calculate the number of DNA molecule copies in a sample based on mass and fragment length.

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ng
0.001 ng1,000,000 ng
bp
1 bp1,000,000,000 bp

Copy Number

9,264,615,385

The estimated number of DNA molecule copies in this sample, using an average of 650 grams per mole per base pair for double-stranded DNA.

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Odeh Ahwal

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Odeh Ahwal
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Frequently Asked Questions

Why is 650 grams per mole per base pair used?

This value represents the average molecular weight of one base pair of double-stranded DNA, accounting for the typical mix of all four nucleotide bases, and is a standard approximation used across molecular biology calculations.

What is this calculation commonly used for?

DNA copy number calculations are routinely used in molecular biology to determine dilutions for PCR reactions, prepare standard curves for quantitative PCR, and calculate molarity for next-generation sequencing library preparation.

Does fragment length need to be exact?

For accurate results, fragment length should reflect the actual measured size of your DNA fragment, often determined by gel electrophoresis or a fragment analyzer, since copy number is inversely proportional to fragment length.

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