qPCR Efficiency Calculator
Chemistry & BiologyCalculate qPCR amplification efficiency and amplification factor from a standard curve slope.
PCR Efficiency
Amplification efficiency as a percentage. A value near 100% indicates the reaction is doubling each cycle.
Amplification Factor
1.988
The factor by which template amount multiplies each cycle. A value near 2 indicates near-perfect doubling.
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Frequently Asked Questions
What is a good qPCR efficiency?
An efficiency between 90% and 110% is generally considered acceptable for a reliable qPCR assay, corresponding to a slope roughly between -3.1 and -3.6.
Where does the slope value come from?
The slope comes from a standard curve: plotting the cycle threshold (Ct) value against the logarithm of starting template concentration across a dilution series, then fitting a straight line through the points.
What does an amplification factor of 2 mean?
An amplification factor of 2 means the amount of PCR product exactly doubles with every cycle, which is the theoretical maximum and corresponds to 100% efficiency.
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